METAWATER

Sensor technologies

Cryptosporidium sensor

Conducting inspections to detect a water-based chlorine-resistant protozoa called "Cryptosporidium" is problematic. Its pretreatment is complicated and time-consuming and the measurement itself is very difficult. Because the judgment of contamination may fluctuate depending on the evaluator, it is difficult in fact to promptly reflect the inspection result in the management of a water treatment process.

To address this challenge, METAWATER is engaged in the development of equipment to automatically measure the Cryptosporidium level in raw water (source water from rivers) for supply water within three hours.

Using a flat film attached to a filtration unit, Cryptosporidium in the sample water are separated and concentrated on the film surface so as to collect them as a concentrated sample. Then, the sample is dyed with a fluorescent-labelled antibody inside the labelling equipment. The number of Cryptosporidium dyed with the fluorescent antibody is counted optically using an optical detector (a flow cytometer dedicated for this purpose). If any Cryptosporidium is detected with the optical detector, the sample should be separated and preserved so that it can be used for detailed inspection such as DNA analysis, etc.

Cryptosporidium sensor

Characteristics

  • Measurement within three hours (can measure three times a day)
  • With addition of a ceramic membrane concentration device, options between raw water (1 L) and treated sedimentation water (10 L) become available in measurement.
  • Any sample judged as positive is separated and preserved. Those samples can be used for more detailed inspections such as microscope inspection or genetic inspection.

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